In vitro skin corrosion

Irreversible damage to the skin is known as corrosion, which can be caused by chemicals and other substances. We investigate corrosive properties without animal testing using the in-vitro method on cultivated, human 3D skin models in accordance with OECD 431. The test substances are applied to the skin models and after a short exposure time the viability of the skin cells is photometrically determined using a biochemical dye. For the evaluation of a corrosive effect, two contact times of the samples with the skin models are examined, whereby a loss of vitality of the skin models of more than 50 % within a short exposure time and more than 85 % with longer exposure is classified as corrosive.