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EN Widerstandsfähigkeit gegenüber Mikroorganismen

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DIN EN ISO 846 – Plastics - Determination of the effect of microorganisms on plastics

Fungi and bacteria that colonize plastic surfaces can degrade the plastic or cause damage to the plastic components through the influence of their metabolic products. Such effects of microorganisms can be detected using the various methods (methods A to C) of DIN EN ISO 846.

In method A, the resistance of a plastic to fungi is tested. A spore suspension of 5 different fungi is dripped onto the material samples and incubated for 4 weeks. If the plastic contains components that can be utilized by the fungus, the material is degraded. In a similar, more advanced method, the fungistatic or fungicidal effect (methods B and B') can be determined. Here, the plastic is placed on a carbon-containing culture medium with fungal spores in order to be able to examine growth inhibition on or around the sample.

Method C is used to determine the resistance of a plastic to bacteria. The samples are embedded in a carbon-free nutrient agar with the bacterium Pseudomonas aeruginosa. Increased growth of the bacterium at the edge of the sample indicates utilization of the material and possible decomposition.


DIN EN 14119 – Testing of textiles - Determination of the effect of microscopic fungi (microfungi)

The standard specifies a method for determining the resistance of textiles to the effects of microfungi. It is applicable to all textile products, including man-made fibers and cellulose-based products. In the course of the test for antifungal efficacy (method B2), test samples are placed on an inoculated complete agar nutrient medium containing fungal spores of the test organisms Aspergillus niger and Chaetomium globosum and incubated for 14 days at a defined temperature and humidity that is optimal for the molds.

A. niger allows the textiles to be assessed with regard to their antifungal properties, while the use of C. globosum serves to prove the protective effect of the textile treatment against cellulose-degrading microfungi. Depending on customer requirements, the selection of test organisms can also be customized. Finally, the effectiveness is evaluated on the basis of a visual assessment of the growth on and under the samples, as well as by measuring a possible fungus-free zone around the test piece.
 

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List of test standards for EN Widerstandsfähigkeit gegenüber Mikroorganismen
DIN EN 14119

Testing of Textiles – Evaluation of the action of microfungi

DIN EN ISO 846

Plastics – Evaluation of the action of microorganism